In this work, we designed a multi-signal readout platform for sensitive monitoring carbamate pesticide based on gold nanoclusters-anchored MnO₂(AuNCs-MnO₂) nanocomposite. The fluorescence of AuNCs was quenched by MnO₂via Forster resonance energy transfer effect. In the presence of acetylcholinesterase and choline oxidase, the substrate acetylcholine (ACh) is catalyzed to generate H₂O₂that can specially induce the decomposition of MnO₂, coupling with change of color and recovery of fluorescence. Carbamate pesticide as an inhibitor of acetylcholinesterase prevent the generation of H₂O₂, further block the breakage of MnO₂, which caused the fluorescence switch. Such color and fluorescence respond can be utilized for highly sensitive carbamate pesticide identification and quantification. Under the optimum conditions, the dual-output assay possessed good sensitivity for rapid detection of carbaryl (model analyte) with a detection limit of 0.125 mu g L^-1. The fluorometric/colorimetric bimodal platform showed good stability and anti-interference ability comparing to that of others enzyme-based sensor.