Rational immobilization of fragile enzymes on a scaffold is an efficient protocol to achieve robust biocatalyst, yet the construction remains challenging due to the trade-off between enzyme activity and framework environment. Herein, an enzyme-engineered assembly strategy enabling the in situ fusion of acetylcholinesterase (AChE) into Zn-based metal-organic frameworks (Zn-MOF-74) during the rapid nucleation and crystal defect growth process, is demonstrated. The Zn-MOF-74 composites can offer a hydrophilic framework microenvironment for maintaining the biological state of AChE, with flower-like porous structure that allows for boosting the mass transfer process of substrates and products. Impressively, the apparent activity of AChE can be well maintained 90% after encapsulation, and the AChE@Zn-MOF-74 composites produced excellent stability in inhospitable environments. Given the structural integration, a robust AChE@Zn-MOF-74-based biosensor is constructed for the sensitive detection of chlorpyrifos (organophosphorus pesticides) with a detection limit of 1.0 ng mL⁻¹, enabling the monitoring of pesticide degradation extent in the water body. This study is anticipated to offer valuable insight into understanding the structure-activity relationship of enzyme@MOF to encourage applications in the construction of robust biosensors for precision agriculture.

原文链接：https://doi.org/10.1002/adfm.202309383